Composite

Part:BBa_K3089035:Design

Designed by: Aiai Dong   Group: iGEM19_Greatbay_SCIE   (2019-10-17)


T7 promoter+csgA-linker-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal PstI site found at 400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 400
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal PstI site found at 400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal PstI site found at 400
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 522


Design Notes

Linkers are attached to prevent each component jamming together sfGFP used as marker for protein expression


Source

CsgA - E.coli MG1655


References